Figure 3.

Indirect alloactivation of naive and memory T cells following coculture with autologous APCs in the presence of sonicates derived from allogeneic EC, fibroblasts or RPTEC. Nave CD45RA+ cells and memory CD45RO+ CD4+ T cells were isolated from PBMC as described in Methods. Panel A, 1x105 CD45RA+CD4+ T cells (white bars) or CD45RO+CD4+ T cells (black bars) were cocultured for 6days with 1x105 irradiated autologous PBMC either alone (striped bars) or in the presence of sonicate (solid bars) prepared from allogeneic IFN treated EC, fibroblasts or RPTEC. Proliferation was assessed by [3H] thymidine incorporation. Results are representative of 3 different experiments performed in triplicate 1SD (*P<0.05). Panel B, 1x105 CD45RA+ (Upper Panel) or CD45RO+ (Lower Panel) CD4+ T cells were cultured for 6days either alone (gray bars) or with sonicate prepared from allogeneic IFN-treated EC (black bars), fibroblasts (striped bars) or RPTEC (checked bars). Proliferation was assessed by [3H] thymidine incorporation. The illustrated results in Panels A and B are representative of 3 different experiments performed in triplicate 1SD

Samsonov et al. Transplantation Research 2012 1:4   doi:10.1186/2047-1440-1-4
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